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Self-assembled hyaluronate/protamine polyelectrolyte nanoplexes: Synthesis, stability, biocompatibility and potential use as peptide carriers
Umerska, A. Paluch, Krzysztof J. Santos Martinez, M.-J. Corrigan, O.I. Medina, C. Tajber, L.
Umerska, A.
Paluch, Krzysztof J.
Santos Martinez, M.-J.
Corrigan, O.I.
Medina, C.
Tajber, L.
Publication Date
2014
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26/11/2013
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Abstract
This work investigates a new type of polyelectrolyte complex nanocarrier composed of
hyaluronic acid (HA) and protamine (PROT). Small (approximately 60 nm) and negatively
charged nanoparticles (NPs) with a polydispersity index of less than 0.2 were obtained with
properties that were dependent on the mixing ratio, concentration of polyelectrolytes and
molecular weight of HA. Salmon calcitonin (sCT) was efficiently (up to 100%) associated
with the NPs, and the drug loading (9.6-39% w/w) was notably high, possibly due to an
interaction between HA and sCT. The NPs released ~70-80% of the sCT after 24 hours, with
the estimated total amount of released sCT depending on the amount of HA and PROT
present in the NPs. The isoelectric point of the NPs was close to pH 2, and the negative
surface charge was maintained above this pH. The HA/PROT nanoplexes protected the sCT
from enzymatic degradation and showed low toxicity to intestinal epithelial cells, and thus
may be a promising oral delivery system for peptides.
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Umerska A, Paluch KJ, Santos Martinez M-J et al (2014) Self-assembled hyaluronate/protamine polyelectrolyte nanoplexes: Synthesis, stability, biocompatibility and potential use as peptide carriers. Journal of Biomedical Nanotechnology. 10(12): 3658-3673.
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