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Antibiotic functionalised polymers reduce bacterial biofilm and bioburden in a simulated infection of the cornea

Doroshenko, N.
; 
Rimmer, Stephen
; 
Hoskins, Richard
; 
Garg, P.
; ; 
Spencer, Hannah L.M.
; 
Lord, Rianne M.
; ; 
Pownall, D.
; 
MacNeil, S.
... show 2 more
Publication Date
2018
End of Embargo
Supervisor
Keywords
Microbial keratitis, Cornea, Corneal trauma, Antimicrobial therapy
Rights
© The Royal Society of Chemistry 2018. Open Access article. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
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Peer-Reviewed
Yes
Open Access status
openAccess
Accepted for publication
17/05/2018
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Department
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Life Sciences Publications
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Abstract
Microbial keratitis can arise from penetrating injuries to the cornea. Corneal trauma promotes bacterial attachment and biofilm growth, which decrease the effectiveness of antimicrobials against microbial keratitis. Improved therapeutic efficacy can be achieved by reducing microbial burden prior to antimicrobial therapy. This paper assesses a highly-branched poly(N-isopropyl acrylamide) with vancomycin end groups (HB-PNIPAM-van), for reducing bacterial attachment and biofilm formation. The polymer lacked antimicrobial activity against Staphylococcus aureus, but significantly inhibited biofilm formation (p = 0.0008) on plastic. Furthermore, pre-incubation of S. aureus cells with HB-PNIPAM-van reduced cell attachment by 50% and application of HB-PNIPAM-van to infected ex vivo rabbit corneas caused a 1-log reduction in bacterial recovery, compared to controls (p = 0.002). In conclusion, HB-PNIPAM-van may be a useful adjunct to antimicrobial therapy in the treatment of corneal infections.
URI
http://hdl.handle.net/10454/16402
Version
Published version
Citation
Doroshenko N, Rimmer S, Hoskins R et al (2018) Antibiotic functionalised polymers reduce bacterial biofilm and bioburden in a simulated infection of the cornea. Biomaterials Science. 6(8): 2101-2109.
Link to publisher’s version
Link to published version
Link to Version of Record
https://doi.org/10.1039/C8BM00201K
Type
Article
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