Nanoparticle labels for pathogen detection through nucleic acid amplification tests

Magnetic nanoparticles and surface-enhanced Raman scattering (SERS) active nanoparticles were coated with short chain DNA tags. These were then used to identify a target bacterial DNA sequence. The tags function as primers in a standard PCR with the reverse primers and forward primers on the SERS nanoparticles and magnetic nanoparticles, respectively. During the PCR cycles, a composite nanostructure is formed that is both magnetically responsive and SERS active. After magnetic trapping, the intensity of the SERS signal can be related back to the concentration of the target DNA. A test assay was performed that showed a detection limit (based on the signal to noise ratio) of less than 3 zeptomole (41 pg/L). For comparison, a PCR assay based on the standard SYBR Green method was performed. This used the same primers and target DNA and had a detection limit of 10 attomoles (138 ng/L), 3,000 times less sensitive. The work documents the proof of principle study and shows for the first time the use of SERS-NP labels in the quantification of nucleic acid amplification tests and PCR.

URI

http://hdl.handle.net/10454/10333

Version

Accepted manuscript

Citation

Drake P, Chen Y-C, Lehmann I et al (2015) Nanoparticle labels for pathogen detection through nucleic acid amplification tests. Microfluidics and Nanofluidics. 19(2): 299-305.

Link to Version of Record

https://doi.org/10.1007/s10404-014-1530-8

Type

Article