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Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures

Chan, K.
; 
Priya, K.
; 
Chang, Chien-Yi
; 
Abdul Rahman, A.Y.
; 
Tee, K.K.
; 
Yin, W.
Publication Date
19/07/2016
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Keywords
Heat shock, RNA sequencing, Pseudomonas aeruginosa PAO1, Transcriptome, Gene expression
Rights
© 2016 Chan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed.
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Yes
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openAccess
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15/06/2016
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Life Sciences Publications
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Abstract
Functional genomics research can give us valuable insights into bacterial gene function. RNA Sequencing (RNA-seq) can generate information on transcript abundance in bacteria following abiotic stress treatments. In this study, we used the RNA-seq technique to study the transcriptomes of the opportunistic nosocomial pathogen Pseudomonas aeruginosa PAO1 following heat shock. Samples were grown at both the human body temperature (37 C) and an arbitrarily-selected temperature of 46 C. In this work using RNA-seq, we identified 133 genes that are differentially expressed at 46 C compared to the human body temperature. Our work identifies some key P. aeruginosa PAO1 genes whose products have importance in both environmental adaptation as well as in vivo infection in febrile hosts. More importantly, our transcriptomic results show that many genes are only expressed when subjected to heat shock. Because the RNA-seq can generate high throughput gene expression profiles, our work reveals many unanticipated genes with further work to be done exploring such genes products.
URI
http://hdl.handle.net/10454/15203
Version
Published version
Citation
Chan K, Priya K, Chang C et al (2016) Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures. PeerJ. 4:e2223.
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Link to Version of Record
https://doi.org/10.7717/peerj.2223
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Article
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