A proteomic investigation of Streptococcus agalactiae grown under conditions associated with neonatal exposure reveals the upregulation of the putative virulence factor C protein β antigen
Yang, Q. Zhang, M. Harrington, Dean J. Black, G.W. Sutcliffe, I.C.
Yang, Q.
Zhang, M.
Harrington, Dean J.
Black, G.W.
Sutcliffe, I.C.
Publication Date
2010-06
End of Embargo
Supervisor
Rights
Peer-Reviewed
Yes
Open Access status
closedAccess
Accepted for publication
2010-01-06
Institution
Department
Awarded
Embargo end date
Collections
Additional title
Abstract
Streptococcus agalactiae is a major neonatal pathogen that is able to adapt to a variety of host environments, including both rectal and vaginal maternal carriage, growth in amniotic fluid and at various neonatal body sites. As such it is important to elucidate the patterns of protein expression that are associated with S. agalactiae growth under these different in vivo conditions. To this end, we have grown S. agalactiae strain A909 under in vitro conditions reflecting those associated with maternal vaginal carriage (low pH, low oxygen, nutrient stress) and those associated with exposure to body fluids during invasive disease (neutral pH, aeration, nutrient sufficient). The protein profiles of bacterial cells grown under each of these conditions were compared using a proteome approach. A total of 76 proteins were reproducibly identified 16 of which were shown to be differentially expressed. The putative virulence factor C protein β and several proteins linked to resistance to oxidative stress were found to be upregulated under the conditions hypothesised to reflect those associated with foetal exposure to S. agalactiae. Thus, these data add to the currently limited understanding of the molecular basis of S. agalactiae GBS adaptation to different environmental conditions.
Version
No full-text in the repository
Citation
Yang Q, Zhang M, Harrington DJ et al (2010) A proteomic investigation of Streptococcus agalactiae grown under conditions associated with neonatal exposure reveals the upregulation of the putative virulence factor C protein β antigen. International Journal of Medical Microbiology. 300(5): 331-337.
Link to publisher’s version
Link to published version
Link to Version of Record
Type
Article